リウマチ

Apoptosis. 2006 Jun;11(6):1049-59.

Oxidative stress regulated expression of ubiquitin Carboxyl-terminal
Hydrolase-L1: role in cell survival.

Shen H, Sikorska M, Leblanc J, Walker PR, Liu QY.

Neurobiology Program, Institute for Biological Sciences, National Research
Council of Canada, Ottawa, Ontario, Canada, K1A 0R6.

The ubiquitin Carboxyl-terminal Hydrolase-L1 gene (UCHL1) is a key enzyme in the
protein degradation pathway; however, its precise role in protecting cells under
stress conditions is unclear. In the present study we investigated the activity
of this gene in human NT2/D1 embryonal carcinoma cells subjected to
oxygen-glucose deprivation (OGD) and reoxygenation. OGD/reoxygenation cause
global metabolic changes due to energy withdrawal and the subsequent generation
of reactive oxygen species which initiates either a stress-adaptation-survival
response or cell death, depending on the severity of the insult. A bi-phasic
change in UCHL1 expression was observed by Q-PCR, Western blotting and flow
cytometry. Down regulation of UCHL1 was detected immediately after OGD treatment
and its expression was subsequently restored and increased 6 h after OGD
treatment as well as during reoxygenation. Furthermore, flow cytometry analysis
detected a lower level of UCHL1 only in apoptotic cells that had severe loss of
mitochondrial membrane potential. Accordingly, down-regulation of endogenous
UCHL1 by antisense cDNA in mouse N2a neuroblastoma cells increased the cell's
sensitivity to OGD treatment. This down-regulation of endogenous UCHL1 led to the
accumulation of p27, suggesting that UCHL1 is an essential gene to maintain cell
homeostasis under normal growth and oxidative stress conditions.
・Caspase-activated DNase (CAD)/DNA fragmentation factor 40kDa (DFF40) = Apoptosisの際のDNA ladder生成に関与
・DNase II (lysozomal) = apoptosis or necrosis の細胞核を取り込んだライソゾーム内でDNAを分解
・軟骨細胞：Chondroprogenitor Clone：ATDC5（mouse）
○　Insulin-Induced Chondrogenesis of ATDC5 Cells
○　iP (20mM); Collgen type II -> type X 肥大軟骨細胞？　アポトーシス？
・eat me signal JCB 155:501,2001
MFG-E8 (milk fat globule EGF factor 8)
Tim-4
・Reiter cells are macrophages containing ingested polymorph nuclei that are commonly found in most inflammatory synovial fluids.